Proregenerative Microenvironment Triggered by Donor Mesenchymal Stem Cells Preserves Renal Function and Structure in Mice with Severe Diabetes Mellitus

Table 1

MSC administration did not improve diabetic condition.

	Before MSC administration		8 weeks after MSC administration
	Normal	DM	Normal	DM	DM + MSCs
Body weight (g)	24.2 ± 0.4	20.2 ± 0.3	26.9 ± 0.5	21.4 ± 0.5	22.2 ± 0.5
Plasma insulin (g/L)	0.61 ± 0.12	0.03 ± 0.01	0.51 ± 0.10	0.07 ± 0.03	0.07 ± 0.03
Blood glucose (mg/dL)	171 ± 2	715 ± 49	183 ± 11	766 ± 68	740 ± 40
Serum cholesterol (mg/dL)	97 ± 10	173 ± 20	146 ± 46	291 ± 32	253 ± 52
Serum triglycerides (mg/dL)	72 ± 14	198 ± 35	97 ± 39	337 ± 55	217 ± 39
Kidney weight (mg)	158 ± 4	217 ± 9	177 ± 5	311 ± 15	278 ± 14
Kidney/body weight (mg/g)	5.7 ± 0.2	8.9 ± 0.3	6.6 ± 0.2	15 ± 0.7	12.6 ± 0.9
Urine volume (mL/24 h)	1.3 ± 0.1	10.0 ± 1.6	2.4 ± 0.9	16.8 ± 2.3	16.3 ± 2.2

Physical and biochemical parameters of experimental animals. Before MSC administration (eight weeks after diabetes induction), mice received 200 μL of 5% mice plasma (DM) or 0.5 10⁶ MSCs resuspended in 200 μL of 5% mice plasma (DM + MSCs) via the tail vein. All biochemical parameters were evaluated in urine and plasma samples obtained after four hours of fasting. Data are presented as mean SEM, . versus Normal; ^# versus DM for the same time point.