Proregenerative Microenvironment Triggered by Donor Mesenchymal Stem Cells Preserves Renal Function and Structure in Mice with Severe Diabetes Mellitus

Table 2

MSC administration prevents renal failure.

	Before MSC administration		2 weeks after MSC administration			8 weeks after MSC administration
	Normal	T1DM	Normal	T1DM	T1DM + MSC	Normal	T1DM	T1DM + MSC
Urinary albumin excretion (U-Alb g/U-Crea mg)	6.4 ± 1.4	35.0 ± 3.1	6.7 ± 1.1	40.2 ± 4.3	27.4 ± 4	7.2 ± 0.8	62.2 ± 9.5	30.9 ± 2.5
Blood urea nitrogen (mg/dL)	30.1 ± 2	40.2 ± 3.3	32 ± 1.8	42.1 ± 2.2	45.5 ± 4.7	31.9 ± 2.5	67.3 ± 3.8	46.5 ± 3.4
Plasma cystatin C (mg/L)	0.07 ± 0.01	0.11 ± 0.01	0.06 ± 0.01	0.11 ± 0.02	0.08 ± 0.01	0.06 ± 0.02	0.13 ± 0.02	0.08 ± 0.01
Plasma creatinine (mg/L)	2.26 ± 0.06	2.29 ± 0.08	2.30 ± 0.07	2.27 ± 0.06	2.30 ± 0.13	2.28 ± 0.07	2.60 ± 0.05	2.29 ± 0.05

Markers of renal function were evaluated before MSC administration (eight weeks after diabetes induction) and after two and eight weeks after MSC administration. All animals were housed in metabolic cages to collect urine for albuminuria determinations; blood was collected and used to measure plasmatic creatinine and urea nitrogen levels. Data are presented as mean SEM, . versus Normal; ^# versus DM for the same time point.