Mass Spectrometry-Based Proteomic Study Makes High-Density Lipoprotein a Biomarker for Atherosclerotic Vascular Disease

Figure 4

MALDI-TOF-MS analysis of human HDL. HDL was dialyzed against degassed 20 mM Tris-HCl, 0.5 mM EDTA, and 0.02% NaN₃, pH 8.0 at 4°C with 3 buffer changes in 24 hours. MALDI-TOF-MS (Ultraflex III TOF/TOF, Bruker Daltonics, Germany) with linear mode was used to identify the major apolipoproteins and their isoforms in HDL. Other detailed experimental settings can be referred to in [16]. ApoC1 (calculated mass: 6630.6 m/z); ApoC1′: ApoC1 minus N-terminus Thr-Pro (calculated mass: 6432.4 m/z); ApoCIII₀ (calculated mass: 8765.7 m/z); ApoAII monomer: single chain ApoAII (calculated mass: 8809.9 m/z); proCII (calculated mass: 8914.9 m/z); SAA4 (calculated mass: 12863.2 m/z); ApoAII′: apoAII minus C-terminus-Gln (calculated mass: 17253.7 m/z); ApoAI (calculated mass: 28078 m/z).