Figure 3: Annexin V FITC/PI staining used to assess the mode of cell death. RFRA (R. fairholmianus root acetone) treated Caco-2 cells showed an increased percentage of apoptotic population after 24 h incubation. The population of early and late apoptotic cells in control group found to be lower (22.3 and 3.3%) compared with experimental groups; the attached table shows the statistical data of flow cytometric analysis.