BioMed Research International / 2015 / Article / Tab 1

Research Article

CK2 Secreted by Leishmania braziliensis Mediates Macrophage Association Invasion: A Comparative Study between Virulent and Avirulent Promastigotes

Table 1

Effect of casein on the secreted CK2 activities of Leishmania braziliensis avirulent and virulent samples.

SystemsVirulent strainAvirulent strain Virulent activity/avirulent
activity
pmol·Pi mg−1·min−1
± E.P.
pmol·Pi mg−1·min−1
± E.P.

Constitutive ecto-CK2 activity (eCK2)34.400 ± 1.3400.040 ± 0.001860.00
Constitutive intracellular CK2 activity (iCK2)4.800 ± 0.6100.290 ± 0.00216.55
Constitutive secreted CK2 activity (CsCK2)7.290 ± 0.3200.060 ± 0.006121.50
Inducible secreted CK2 activity (IsCK2)13.960 ± 1.010*0.070 ± 0.006199.43

The reactions were performed as described in Section 2. The values represent the mean of 3 independent experiments conducted in triplicate (* indicates P ≤ 0.005 in relation to CsCK2; one-way ANOVA, Tukey’s post hoc test).

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