BioMed Research International / 2015 / Article / Tab 2

Research Article

CK2 Secreted by Leishmania braziliensis Mediates Macrophage Association Invasion: A Comparative Study between Virulent and Avirulent Promastigotes

Table 2

Secreted kinase activity of Leishmania braziliensis promastigotes using different substrates.

SubstratesSecreted kinase activity 
(pmol·Pi mg−1·min−1)
Percentage (%)aPercentage (%)b

Casein7.282 ± 0.476100
CK2-specific Peptide5.790 ± 0.24579.510100
CK2-specific peptide + heparin0.327 ± 0.210*4.4905.648

The reactions were performed as described in Section 2. The values are presented as the mean ± SEM of at least 3 independent experiments, which were performed in triplicate (*P < 0.05 in relation to CK2-specific peptide; one-way ANOVA, Tukey’s post hoc test).
aThe kinase activity was arbitrarily considered to be 100% when the value was obtained using casein as substrate. Based on the secreted kinase activity using CK2-specific peptides, the percentage was estimated by comparison with that obtained using casein, whereas the activity sensitive to heparin (1 μg/mL) was considered CK2 activity.
bThe kinase activity was arbitrarily considered to be 100% when the value was obtained using the CK2 peptide as substrate.

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