|The reactions were performed as described in Section 2. The values are presented as the mean ± SEM of at least 3 independent experiments, which were performed in triplicate (*P < 0.05 in relation to CK2-specific peptide; one-way ANOVA, Tukey’s post hoc test).|
aThe kinase activity was arbitrarily considered to be 100% when the value was obtained using casein as substrate. Based on the secreted kinase activity using CK2-specific peptides, the percentage was estimated by comparison with that obtained using casein, whereas the activity sensitive to heparin (1 μg/mL) was considered CK2 activity.
bThe kinase activity was arbitrarily considered to be 100% when the value was obtained using the CK2 peptide as substrate.