CK2 Secreted by Leishmania braziliensis Mediates Macrophage Association Invasion: A Comparative Study between Virulent and Avirulent Promastigotes

Table 2

Secreted kinase activity of Leishmania braziliensis promastigotes using different substrates.

Substrates	Secreted kinase activity; (pmol·Pi mg^−1·min^−1)	Percentage (%)^a	Percentage (%)^b
Casein	7.282 ± 0.476	100	—
CK2-specific Peptide	5.790 ± 0.245	79.510	100
CK2-specific peptide + heparin	0.327 ± 0.210^*	4.490	5.648

The reactions were performed as described in Section 2. The values are presented as the mean ± SEM of at least 3 independent experiments, which were performed in triplicate (^*P < 0.05 in relation to CK2-specific peptide; one-way ANOVA, Tukey’s post hoc test).
^aThe kinase activity was arbitrarily considered to be 100% when the value was obtained using casein as substrate. Based on the secreted kinase activity using CK2-specific peptides, the percentage was estimated by comparison with that obtained using casein, whereas the activity sensitive to heparin (1 μg/mL) was considered CK2 activity.
^bThe kinase activity was arbitrarily considered to be 100% when the value was obtained using the CK2 peptide as substrate.