BioMed Research International / 2015 / Article / Tab 3

Research Article

CK2 Secreted by Leishmania braziliensis Mediates Macrophage Association Invasion: A Comparative Study between Virulent and Avirulent Promastigotes

Table 3

Effect of polyamines on the constitutive and inducible secreted CK2 activity of the Leishmania braziliensis virulent strain.

AdditionConstitutive secreted CK2Inducible secreted CK2
nmol Pi/mg·min−1
% kinase activitynmol Pi/mg·min−1
% kinase activity

None7.70 ± 0.63100.0013.96 ± 0.64100.00
Putrescine (500 μM)14.48 ± 0.52**188.0121.50 ± 1.15**154.01
Spermidine (500 mM)18.00 ± 0.84**233.7725.32 ± 0.24*181.38
Spermine (500 mM)26.75 ± 0.72*347.4032.76 ± 0.26*234.67

The reactions were performed as described in Section 2. The values are presented as the mean of 3 independent experiments conducted in triplicate (* indicates P ≤ 0.0002, ** indicates P ≤ 0.007, and *** indicates P ≤ 0.05 compared with control without addition; one-way ANOVA, Tukey’s post hoc test).

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