Adventitial Alterations Are the Main Features in Pulmonary Artery Remodeling due to Long-Term Chronic Intermittent Hypobaric Hypoxia in Rats

Figure 5

Adventitia and inflammatory cells: (a) density of adventitial cells positive for CD11b (expressed as N° of cells/cm³) and (b) representative confocal images obtained by reconstruction of the adventitia of arteries stained with the nuclear dye DAPI (405 nm excitation/410–475 nm emission) and the specific antibody CD11b and Alexa Fluor 488 secondary antibody (488 nm excitation/500–600 nm emission). Serial images of the adventitia of arteries from rats exposed to CIH2x2, CH, and NX were captured at both wavelengths with a 63x objective zoom. Results are expressed means ± SEs. Statistical analysis was performed using one-way ANOVA.   is exposed versus NX and ^† CH versus CIH2x2.

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