Research Article
Missense Mutations in Exons 18–24 of EGFR in Hepatocellular Carcinoma Tissues
Table 1
Antibodies, dilution, antigen retrieval method, and incubation time of different biomarkers.
| Marker | Antibody | Dilution | Antigen retrieval method | Incubation time |
| EGFR | Mouse monoclonal antibody, clone 111.6, Lab Vision | 1 : 400 | Proteinase XXV (1 mg/mL PBS), 15 min at 37°C | 2 h, RT | P53 | Rabbit monoclonal antibody, clone Y5, Lab Vision | 1 : 100 | 10 mM citrate buffer pH 6.0, pressure cooker | 30 min, RT | Ki-67 | Rabbit monoclonal antibody, clone SP6, Lab Vision | 1 : 200 | 10 mM citrate buffer pH 6.0, pressure cooker | 30 min, RT |
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