Table 1: Antibodies, dilution, antigen retrieval method, and incubation time of different biomarkers.

MarkerAntibodyDilutionAntigen retrieval methodIncubation time

EGFRMouse monoclonal antibody, clone 111.6, Lab Vision1 : 400Proteinase XXV (1 mg/mL PBS), 15 min at 37°C2 h, RT
P53Rabbit monoclonal antibody, clone Y5, Lab Vision1 : 10010 mM citrate buffer pH 6.0, pressure cooker30 min, RT
Ki-67Rabbit monoclonal antibody, clone SP6, Lab Vision1 : 20010 mM citrate buffer pH 6.0, pressure cooker30 min, RT