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BioMed Research International
Volume 2015, Article ID 176582, 6 pages
Research Article

Activity of α1-Antitrypsin and Some Lysosomal Enzymes in the Blood Serum of Patients with Chronic Obstructive Pulmonary Disease after Smoking Cessation

1Department of Neurosurgery, Stanisław Staszic Specialist Hospital, Rydygiera 1, 64-920 Piła, Poland
2The Chair of Medical Biology, Collegium Medicum of Nicolaus Copernicus University, Karłowicza 24, 85-092 Bydgoszcz, Poland
3Dental Medical Clinic Demeter, Białostocka 7, 03-741 Warszawa, Poland
4Family Medicine Center, Przesmyk 2/4, 87-100 Toruń, Poland
5Department of Normal Anatomy, Collegium Medicum of Nicolaus Copernicus University, Łukasiewicza 1, 85-801 Bydgoszcz, Poland

Received 8 October 2014; Revised 20 December 2014; Accepted 8 January 2015

Academic Editor: Nikolaos Siafakas

Copyright © 2015 Bartosz Woźniak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The activity of α1-antitrypsin (AAT) and the lysosomal enzymes, cathepsin D (CTS D), arylsulfatase (ASA), and acid phosphatase, (AcP) was determined in patients with COPD (GOLD category A). Moreover, the diagnostic usefulness of these parameters in blood serum was assessed along with establishing whether smoking cessation affects these parameters. The study included 70 patients with COPD who ceased smoking (study group) and two control groups of 33 subjects each: nonsmokers without COPD (control I) and patients with COPD who continued smoking (control II). In control I, blood was taken once and in control II, at the start of the experiment and after the 1st, 2nd, and 3rd months. AAT in the patients exhibited higher activity than in the healthy subjects at all time points. AAT activity in the patients before the start of the experiment was 80% higher () than in control I. No statistically significant differences in CTS D, ASA, and AcP activity were found. COPD involves increased AAT activity and unchanged activities of the assessed lysosomal enzymes. Three-month tobacco abstinence does not affect these parameters in peripheral blood. Determining the AAT levels in blood serum can be used in the diagnostics of COPD.