Establishment of Hairy Root Cultures of Rhaponticum carthamoides (Willd.) Iljin for the Production of Biomass and Caffeic Acid Derivatives

Table 2

Specific pairs of primers used for PCR analysis of transformed roots of R. carthamoides.

Gene	Primer sequences	Amplified fragments (bp)
rolA	5′ CTA AGG TCA AGA AGA AGA AGG 3′; 5′ AGA AGT TAT AGC CAG AGG AGT 3′	107
rolB	5′ GCT CTT GCA GTG CTA GAT TT 3′; 5′ GAA GGT GCA AGC TAC CTC TC 3′	386
rolC	5′ CTC CTG ACA TCA AAC TCG TC 3′; 5′ TGC TTC GAG TTA TGG GTA CA 3′	582
rolD	5′ GAT GAT TTT CGT TTT ATC AAG 3′; 5′ GAA GGA CAG AGG ATA CTT AAA C 3′	204
aux1	5′ ATC TTA GTC ACT TCA TAG CAG TT 3′; 5′ CTT TTT GAG ATA GAA GAA CAA G 3′	500
aux2	5′ ATA TCT GCT TCA ACA AAA GTA AC 3′; 5′ TGA GTT AAT CGA AAC GAT AAT A 3′	774
virG	5′ AGT TCA ATC GTG TAC TTT CCT 3′; 5′ CTG ATA TTC AGT GTC CAG TCT 3′	319