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BioMed Research International
Volume 2015, Article ID 182418, 7 pages
http://dx.doi.org/10.1155/2015/182418
Research Article

Evaluation of APP695 Transgenic Mice Bone Marrow Mesenchymal Stem Cells Neural Differentiation for Transplantation

1Department of Neurology, The Fifth People’s Hospital of Chongqing, Chongqing 400062, China
2Department of Neurology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China
3Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China

Received 4 December 2014; Accepted 16 March 2015

Academic Editor: Hari Shanker Sharma

Copyright © 2015 Qian Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. Even though there is a therapeutic potential to treat Alzheimer’s disease (AD) with neural cell replenishment and replacement, immunological rejections of stem cell transplantation remain a challenging risk. Autologous stem cells from AD patients however may prove to be a promising candidate. Therefore, we studied the neuronal differentiation efficiency of bone marrow mesenchymal stem cells (MSCs) from APP695 transgenic mice, which share features of human AD. Method. Cultured MSCs from APP695 transgenic mice are used; neuronal differentiation was assessed by immunocytochemistry and Western blot. Correlation with Notch signaling was examined. Autophage flux was assessed by western blot analysis. Results. MSCs from APP695 mice have higher neuronal differentiation efficiency than MSCs from wild type mice (WT MSCs). The expression of Notch-1 signaling decreased during the differentiation process. However, autophagy flux, which is essential for neuronal cell survival and neuronal function, was impaired in the neuronally differentiated counterparts of APP695 MSCs (APP695 MSCs–n). Conclusion. These results suggested autologous MSCs of APP690 mice may not be a good candidate for cell transplantation.