MET Expression in Primary and Metastatic Clear Cell Renal Cell Carcinoma: Implications of Correlative Biomarker Assessment to MET Pathway Inhibitors

Figure 4

Quantitative immunofluorescent staining of MET in matched primary and metastatic cores. We utilized MET4 antibody to determine expression of MET in a cohort of matched primary and metastatic RCC cases. Cytokeratin (Cy2 signal) was utilized to create a tumor mask. DAPI was used to identify the nuclei. MET signal was visualized by Cy5 and intensities of MET expression were measured within the cytokeratin mask, within the histospot in a quantitative fashion.