Genotoxic Effect in Autoimmune Diseases Evaluated by the Micronucleus Test Assay: Our Experience and Literature Review

Figure 1

(a) Buccal mucosa cell with normal nuclei and (b) micronucleated cell from buccal mucosa. Photomicrographs stained with acridine orange viewed at 1000 magnification under fluorescence with an IVFL filter (450–490 nm). Binocular Microscope Carl Zeiss (Axiostar Plus). Sample was collected after a gentle swab of both cheeks oral mucosa with a polished slide; then the samples were spread directly in two separated slides previously identified, fixed with 80% ethanol, and stained for analysis; the sample must contain between 500 and 4000 cells to proceed with MN quantification [16, 17].

(a)

(b)