The Role and Mechanism of α-Klotho in the Calcification of Rat Aortic Vascular Smooth Muscle Cells

Figure 4

(a) Representative BMP2, Runx2, and β-catenin expression levels from VSMCs following treatment with the control, β-GP, β-GP + LiCl, β-GP + rmKlotho, or β-GP + rmKlotho + LiCl media for 12 d. BMP2, Runx2, and β-catenin expression was analyzed by western blotting using GAPDH as the loading control. The quantification of the expression levels of (b) β-catenin, (c) Runx2, and (d) BMP2 is represented as the mean with the SE ( per group) for each group in its respective column. versus control group, versus β-GP group, and versus β-GP + Klotho group.

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