BioMed Research International / 2015 / Article / Tab 2

Review Article

Research Progress on the Role of ABC Transporters in the Drug Resistance Mechanism of Intractable Epilepsy

Table 2

Immunohistochemical localization of P-gp in rat brains: comparison of different fixation and staining protocols.

Protocol Pretreatment (ethanol/acetate)AntibodyControl ratsRats after kainate induced SE
AstrocytesEndothelial cellsNeuronsAstrocytesEndothelial cellsNeurons

#1 (acetone fixed cryostat sections of snap-frozen tissue)C219++(+)
mdr-(Ab-1)+++
H241(+)+(+)

#2 (paraformaldehyde-fixed cryosections)C219(+)(+)
mdr-(Ab-1)(+)(+)+(+)
H241(+)(+)
+C219++
+mdr-(Ab-1)+(+)+
+H241++

#3 (paraformaldehyde-fixed vibratome sections)C219+
mdr-(Ab-1)(+)(+)+(+)
H241(+)
+C219++
+mdr-(Ab-1)+(+)+
+H241++

Consistent immunostaining of P-gp in a specific cell type is indicated by +, weak staining by (+), and a lack of staining by −.