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BioMed Research International
Volume 2015, Article ID 197183, 12 pages
http://dx.doi.org/10.1155/2015/197183
Research Article

A Therapeutic Strategy for Spinal Cord Defect: Human Dental Follicle Cells Combined with Aligned PCL/PLGA Electrospun Material

Xinghan Li,1,2,3 Chao Yang,1,2 Lei Li,1,2,3 Jie Xiong,1,2,3 Li Xie,1,2 Bo Yang,1,2,3 Mei Yu,1,2 Lian Feng,1,2 Zongting Jiang,1,2 Weihua Guo,1,2,4 and Weidong Tian1,2,3

1State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China
2National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China
3Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China
4Department of Pedodontics, West China School of Stomatology, Sichuan University No. 14, 3rd Section, Ren Min Nan Road, Wuhou District, Chengdu 610041, China

Received 25 June 2014; Revised 7 November 2014; Accepted 13 November 2014

Academic Editor: Jun Liao

Copyright © 2015 Xinghan Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Stem cell implantation has been utilized for the repair of spinal cord injury; however, it shows unsatisfactory performance in repairing large scale lesion of an organ. We hypothesized that dental follicle cells (DFCs), which possess multipotential capability, could reconstruct spinal cord defect (SCD) in combination with biomaterials. In the present study, mesenchymal and neurogenic lineage characteristics of human DFCs (hDFCs) were identified. Aligned electrospun PCL/PLGA material (AEM) was fabricated and it would not lead to cytotoxic reaction; furthermore, hDFCs could stretch along the oriented fibers and proliferate efficiently on AEM. Subsequently, hDFCs seeded AEM was transplanted to restore the defect in rat spinal cord. Functional observation was performed but results showed no statistical significance. The following histologic analyses proved that AEM allowed nerve fibers to pass through, and implanted hDFCs could express oligodendrogenic lineage maker Olig2 in vivo which was able to contribute to remyelination. Therefore, we concluded that hDFCs can be a candidate resource in neural regeneration. Aligned electrospun fibers can support spinal cord structure and induce cell/tissue polarity. This strategy can be considered as alternative proposals for the SCD regeneration studies.