A Therapeutic Strategy for Spinal Cord Defect: Human Dental Follicle Cells Combined with Aligned PCL/PLGA Electrospun Material

Figure 3

Comparison of random electrospun PCL/PLGA material and aligned electrospun PCL/PLGA material. (a, d) SEM for random (a) and aligned (d) electrospun PCL/PLGA material. FFT transformation was performed for random (g) and aligned (h) fibers. There was obvious diversity of their directional properties. (b, c, e, f) hDFCs seeded on the electrospun material: (b, e) for 3 days, (c, f) for 5 days, (b, c) for being seeded on random electrospun PCL/PLGA material, and (e, f) for being seeded on aligned electrospun PCL/PLGA material. hDFCs could stretch along the direction of fibers as time went on. (i) HE stained for the sections of hDFCs seeded aligned electrospun PCL/PLGA material. (j, k) Spectral intensity distribution at 0–360-degree radius direction verified the diversity between random electrospun PCL/PLGA material (j) and aligned electrospun PCL/PLGA material (k). Intensity was expressed by summating gray value at 0–360-degree radius direction on a circular range of FFT transformation image (circular center coincided with image center). (j) Random electrospun PCL/PLGA material and (k) aligned electrospun PCL/PLGA material. Scale bar = 100 μm.

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