Cytotoxicity of Cyclodipeptides from Pseudomonas aeruginosa PAO1 Leads to Apoptosis in Human Cancer Cell Lines

Figure 2

Effect of cyclodipeptides from Pseudomonas aeruginosa on HeLa and Caco-2 cell viability. HeLa and Caco-2 cells were incubated in CM medium containing the CDP mix for 24 h. (a) Viability was determined by the MTT assay and quantitation of fluorescence. Bars represent the mean value ± the standard error (SE) of three independent experiments. One-way analysis of variance was carried out, with Tukey’s post hoc test; . Values for SE () are shown in lowercase letters. (b) Nonlinear regression analysis of dose-response for the inhibition of viability by the CDP mix; 95% confidence interval, . HeLa: 50% inhibitory concentration (IC₅₀) = 0.53 mg/mL; = 0.96. Caco-2: IC₅₀ = 0.66 mg/mL, = 0.93.

(a)

(b)