Intratracheal Bleomycin Aerosolization: The Best Route of Administration for a Scalable and Homogeneous Pulmonary Fibrosis Rat Model?

Figure 2

Evolution of myofibroblast number after BLM instillation. (a) Representative fields of lung sections from sham and BLM rats 3, 7, 14, 21, and 56 days after instillation. The immunohistochemistry was performed using an anti-αSMA (smooth muscle actin) antibody (bruin staining) and countercolored with Hemalun and Luxol blue (blue staining). (b) Average number of αSMA-positive (α-SMA⁺) cells in lung sections from sham (in white) and BLM (in grey; per time-point) rats at 3, 7, 14, 21, and 56 days after instillation. versus Sham; ANOVA one way followed by Duncan’s Test. versus 14 d; ANOVA one way followed by Duncan’s Test.

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