Intratracheal Bleomycin Aerosolization: The Best Route of Administration for a Scalable and Homogeneous Pulmonary Fibrosis Rat Model?

Figure 6

Difference between left and right lung fibrotic lesions after BLM aerosolization or instillation. This graph represents the modified Ashcroft score absolute difference between L and R lungs, for control and sham animals (CTL + sham; in white) and after BLM aerosolization (Aerosol., in grey) or instillation (Instil., in dark grey) at all time-points. Quantification was made using the MA-method by two observers. Each circle represents the mean between values obtained by both observers for each animal. The threshold was fixed to include 90% of the values of the CTL + sham group (confidence interval calculated as mean ± 1.66 SEM). 16% and 33% of the values are above this threshold for instilled and aerosolized animals, respectively. CTL + sham: ; BLM Instil.: ; BLM Aerosol.: .