Genome-Wide Methylome Analyses Reveal Novel Epigenetic Regulation Patterns in Schizophrenia and Bipolar Disorder

Figure 5

DNA methylation code and gene expression. (a) The global view of DNA methylation code and gene expression. The heat map is based on levels of gene expression and DNA methylation at each gene locus; a key to the color coding is presented below the map. The table on the left designates the pattern of aberrant promoter methylation and the CpG ratios. (b) HCP methylation is associated with gene repression. Gene Set Enrichment Analysis (GSEA) of HCPs hypermethylated in SZ was performed on expression data from RNA-Seq. The correlation between gene expression and promoter methylation is . (c) DNA methylation in the Npas1 gene promoter in 6 SZ and normal samples. Promoter regions are marked with pink bars and CGIs with green. The blue rectangle with dashed lines indicates the DMR regions: red, SZ samples; green, normal control. (d) Npas1 gene expression in normal (blue) and SZ (dark red) samples. (e) Hypomethylation of promoter 3′-shores of CGIs is associated with increased gene expression ( value < 0.040). (f) DNA methylation in the Hnrnpa1 gene promoter. Promoter regions are marked with pink bars and CGIs with green. The blue rectangle with dashed lines indicates the DMR regions: dark red, SZ samples; blue, normal control. (g) Hnrnpa1 gene expression in normal (blue) and SZ (dark red) samples.

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