Figure 4: Cell apoptosis detection by flow cytometry and Western blotting assays. ((a) and (b)) HTR-8/SVneo cells transfected with pEGFP-DCN showed a significantly higher rate of apoptosis by flow cytometry. ((c) and (d)) JEG-3 cells transfected with plasmid overexpressing decorin showed a significant increase in apoptotic rate as compared to that of the empty vector as demonstrated by flow cytometry. ((e) and (f)) Western blotting analysis of apoptotic protein in cells transfected with pEGFP-DCN displayed an increase of cleaved Caspase-3 (17KD), while it displayed a reduction of Bcl-2 (27KD). (Values are mean ± SEM; ; .)