Biochemical and Molecular Phylogenetic Study of Agriculturally Useful Association of a Nitrogen-Fixing Cyanobacterium and Nodule Sinorhizobium with Medicago sativa L.

Table 5

BLAST results obtained by querying the 16S–23S rRNA gene cluster of Nostoc sp. PTV with GenBank and geographical and ecological origins of the hits.

Closest GenBank relative	GenBank access number	Query coverage, %	Score, %	Identity, %	value	Origin of the strain and reference
Nostoc sp. HA4355-MV2 clone p9D	HQ847576	98	2872	97	0.0	Maniniholo Cave wall, near Haena; USA: Kauai, Hawaii
Nostoc muscorum CCAP 1453/22	HF678509	98	2531	93	0.0	Scottish Association for Marine Science, Molecular and Microbial Biology, Dunstaffnage Marine Laboratory, Oban, PA37 1QA, United Kingdom
Anabaena variabilis ATCC 29413	CP000117	98	2457	92	0.0	It has been studied extensively for over 40 years and is the strain of choice for many laboratories throughout the world
Nostoc sp. PCC 7120	BA000019	98	2453	92	0.0	Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. (Nostoc) strain PCC 7120 is available
Nostoc commune NC1 clone 10	EU586726	98	2441	91	0.0	John Carroll University, 20700, North Park Boulevard, University Heights, OH 44118, USA
Nostoc commune NC1 clone M2	EU586725	98	2441	91	0.0	John Carroll University, 20700, North Park Boulevard, University Heights, OH 44118, USA
Nostoc commune NC5 clone 10	EU586727	98	2437	91	0.0	Biology, John Carroll University, 20700, North Park Boulevard, University Heights, OH 44118, USA
Nostoc commune NC1	EU784149	98	2428	91	0.0	Nostoc commune NC1 was isolated from soil (subaerophyt) in Třeboň/Czech republic in 2006.
Nostoc commune NC5 clone 11	EU586728	98	2423	91	0.0	Biology, John Carroll University, 20700, North Park Boulevard, University Heights, OH 44118, USA
Nostoc ellipsosporum CCAP 1453/15	HE975023	94	2399	92	0.0	Scottish Association for Marine Science, Molecular and Microbial Biology, Dunstaffnage Marine Laboratory, Oban PA37 1QA, United Kingdom
Nostoc cf. punctiforme Bashkir clone 6A	EU586732	96	2378	92	0.0	John Carroll University, 20700, North Park Boulevard, University Heights, OH 44118, USA
Calothrix sp. HA4356-MV2 clone p8i	JN385289	98	2361	90	0.0	Cave wall scraping, Maniniholo Cave near Haena, USA: Kauai, Hawaii
Calothrix sp. HA4340 LM2	KF417425	98	2361	90	0.0	Cave, USA: Kauai, Hawaii, Maniniholo Cave
Anabaena sp. CCAP 1403/4A	HE975015	93	2360	92	0.0	Scottish Association for Marine Science, Molecular and Microbial Biology, Dunstaffnage Marine Laboratory, Oban PA37 1QA, United Kingdom
Nostoc muscorum CCAP 1453/8	HF678508	93	2358	92	0.0	United Kingdom: Scotland
Calothrix sp. HA4356-MV2 clone p8i	JN385289	98	2356	90	0.0	Cave wall scraping, Maniniholo Cave near Haena, USA: Kauai, Hawaii
Cylindrospermum moravicum CCALA 993 clone operon 1	KF052607	97	2331	94	0.0	Cave sediment, Czech Republic: Amaterska Cave, South Moravia
Nostoc punctiforme PCC 73102	CP001037	97	2331	91	0.0	A symbiont from a cycad
Nostoc sp. Peltigera malacea DB3992 cyanobiont	JX219483	97	2322	91	0.0	Cyanobiont of lichenized fungi Peltigera malacea, Iceland
Nostoc muscorum CCAP 1453/20	HF678506	95	2318	91	0.0	Scottish Association for Marine Science, Molecular and Microbial Biology, Dunstaffnage Marine Laboratory, Oban PA37 1QA, United Kingdom
Nostoc sp. 10Dp66E	JQ259187	98	2318	90	0.0	From association with Dynamena  pumila L., White Sea, Russia
Cylindrospermum catenatum CCALA 999 clone operon 1	KF052615	97	2309	94	0.0	Soil, Slovakia: forest above Stara Brzotinska Cave, Slovak Karst
Trichormus anomalus HA4352 LM2	KF417426	96	2309	92	0.0	Cave, USA: Kauai, Hawaii, Maniniholo Cave
Cylindrospermum sp. HA4236-MV2 clone p4	JN385290	98	2309	89	0.0	Taro field, Makiki Nature Center, USA: Oahu, Hawaii
Cylindrospermum  catenatum CCALA 996 clone operon 1	KF052611	97	2307	94	0.0	Soil, Czech Republic: Amaterska Cave, South Moravia
Tolypothrix campylonemoides FI5-MK38 clone p10D	JQ083654	98	2305	90	0.0	Sand, USA: Fort Irwin NTC, San Bernardino Co., California
Cylindrospermum catenatum CCALA 990 clone operon 1	KF052601	95	2302	94	0.0	Soil, Czech Republic: Benešov nad Černou, South Bohemia
Spirirestis rafaelensis WJT-71-NPBG6 clone p1B	JQ083656	98	2300	90	0.0	Joshua Tree National Park, USA: Joshua Tree Forest, San Bernardino Co., California
Cylindrospermum badium CCALA 1000 clone operon 1	KF052616	97	2298	94	0.0	Reclaimed coal mine soil, USA: Pyramid State Recreation Area, Illinois
Cylindrospermum catenatum CCALA 991 clone operon 1	KF052603	97	2293	94	0.0	Soil, Czech Republic: Most Region, North Bohemia
Spirirestis rafaelensis WJT-71-NPBG6 clone p1A	JQ083655	98	2291	89	0.0	Joshua Tree National Park, USA: Joshua Tree Forest, San Bernardino Co., California
Nostoc sp. Peltigera membranacea cyanobiont N6	JX975209	97	2289	91	0.0	Symbiont of Peltigera membranacea lichen, Iceland
Cylindrospermum pellucidum CCALA 992 clone operon 1	KF052605	97	2287	94	0.0	Cave sediment, Slovakia: Dlha chodba in Domica Cave system, Slovak Karst
Hassallia sp. EM2-HA1 clone p4B	HQ847555	98	2282	89	0.0	Soil, Mojave National Preserve, USA: San Bernardino Co., California
Tolypothrix tenuis f. terrestris UFS-BI-NPMV-1A2-F06 clone p13E	JQ083651	98	2277	89	0.0	Arid soil after a burn, foothills of the Onaquee Mts. USA: Utah
Nostoc cf. commune 257-16	HQ877826	96	2271	90	0.0	Subaerial, on Bonampak's archeological building walls, Mexico: Chiapas
Hassallia sp. CNP3-B3-C04 clone p5D	HQ847556	98	2271	89	0.0	Soil, Needles District, Virginia Park, Canyonlands National Park, USA: San Bernardino Co., California
Uncultured cyanobacterium clone Emix3.12	JX887892	92	2269	91	0.0	Freshwater microbial mat; Konstanz, Germany
Cylindrospermum muscicola SAG 44.79 clone operon 1	KF111150	96	2268	93	0.0	Soil; France: Gif-Sur-Yvette, Ile-de-France Region
Tolypothrix tenuis f. terrestris UFS-BI-NPMV-1A2-F06 clone p13F	JQ083652	98	2268	89	0.0	Arid soil after a burn, foothills of; the Onaquee Mts.; USA: Utah
Hassallia sp. CM1-HA11 clone p8A	JQ083650	98	2268	89	0.0	Sandy loam near gypsum mine,; USA: Clark Mountains, San Bernardino Co.,; California
Hassallia sp. CM1-HA08 clone p7B	JQ083648	98	2268	89	0.0	Sandy loam near gypsum mine,; USA: Clark Mountains, San Bernardino Co.,; California
Nostoc cf. commune 257-20	HQ877827	94	2266	91	0.0	Biofilms of N. cf. commune were collected at Bonampak archeological area in 2008 from two sites on the building walls (Chiapas, Mexico).
Tolypothrix campylonemoides FI5-MK38 clone p10A	JQ083653	98	2266	89	0.0	Sand; USA: Fort Irwin NTC, San Bernardino Co.,; California
Hassallia sp. CM1-HA08 clone p7F	HQ847554	98	2266	89	0.0	Soil, Clark Mountains, near gypsum; mine; USA: San Bernardino Co., California
Desmonostoc sp. HA7617 LM4	KF417429	96	2241	90	0.0	USA: Kauai, Hawaii, Waikapalae Cave
Camptylonemopsis sp. HA4241-MV5 clone B2-3 + p4	JN385292	96	2223	93	0.0	Moleka stream; USA: Oahu, Hawaii
Anabaena circinalis 33-10 isolate	EF634474	98	2199	88	0.0	Ohau Channel, New Zealand
Tolypothrix sp. PCC 7504 isolate DBSU 18	FJ660999	90	2194	92	0.0	Freshwater; Aquarium, Sweden
Nostoc sp. UAM 307	HM623782	70	2111	98	0.0	Rock surface of calcareous river; Spain: Matarranya River, Teruel, East Spain
Rivularia sp. 1PA23	FJ660980	92	2021	88	0.0	Pozas Azules I, Mexico; Microbialite; freshwater
Nostoc sp. 8964:3	AM711541	66	2006	99	0.0	Host is Gunneraprorepens (Angiospermae), New Zealand
Rivularia sp. 1PA21	FJ660978	92	2004	88	0.0	Institute of Ecology,; UNAM (Mexico)
Nostoc linckia var. arvense IAM M-30	AB325907	65	1999	99	0.0	Cultivated samples from the Institute of; Molecular Biosciences at the University of Tokyo
Aphanizomenon ovalisporum ILC-164	JF768745	92	1988	93	0.0	Lake Kinneret, Israel; Banker et al., 1997 [5]
Nostoc muscorum Ind33	HM573462	65	1988	99	0.0	Paddy field,; India: Agricultural Farms, Banaras Hindu University, Varanasi, Uttar Pradesh