Effects of Arsenite Resistance on the Growth and Functional Gene Expression of Leptospirillum ferriphilum and Acidithiobacillus thiooxidans in Pure Culture and Coculture

Table 1

Specific primers used for general and real-time PCR.

Primer	Primer sequences (5′-3′)	Temperature (°C)	Amplicon length (bp)	qPCR quantification target gene and function
Lf(YSK)-F; Lf(YSK)-R	GAAAACACTTGAGGACGG; CGGATAAAACGGTTGATT	56; 56	168	gyrB gene of L. ferriphilum YSK
At(A01)-F; At(A01)-R	GACCCGTACCCTCAATCA; CGGTTTCACTTCACTGGA	58; 58	175	gyrB gene of At. thiooxidans A01
Lf-ArsB-F; Lf-ArsB-R	GGAGGCAAGGTCTGCGAGAA; GGGAAAAGGGCGGCTCATTC	62; 65	138	arsB gene of L. ferriphilum YSK, membrane-associated arsenite export pump
Lf-ArsU-F; Lf-ArsU-R	GCCAATCCGGTCCTGGTTCA; CCCATTTTCTTTTCGGGCTTTC	65; 64	146	arsU gene of L. ferriphilum YSK, arsenic metabolism related protein of unknown function
Lf-ArsRC-F; Lf-ArsRC-R	GAACGACAGGCATTTTGAGT; GACGAGGACCTTTCTGACCA	55; 57	138	arsRC gene of L. ferriphilum YSK, arsenate reductase/phosphotyrosine protein phosphatase gene
At-ArsB-F; At-ArsB-R	AAAAGTCGCTGTGGGTGAAA; GTTGGGTGCTTGTATTGCTG	58; 56	112	arsB gene of At. thiooxidans A01, arsenical pump membrane protein
At-ArsC-F; At-ArsC-R	ATGGCGGGTCCGAGGTAGGC; GAGGGGTTTGCCACGGAAGG	67; 66	128	arsC gene of At. thiooxidans A01, arsenate reductase
At-ArsH-F; At-ArsH-R	TCCGACCATTCTACCAGTTCC; AGCGGCTATTACGCCATTTT	58; 59	127	arsH gene of At. thiooxidans A01, arsenical resistance protein
At-ArsR-F; At-ArsR-R	ACTAATGCCCGAACTACGCT; TATTGCCGAACATCTGGACC	58; 56	141	arsR gene of At. thiooxidans A01, transcriptional regulator, a negative regulator of the ars operon