A Complex Genome-MicroRNA Interplay in Human Mitochondria

Figure 13

Luciferase assay of hsa-miR-mit3 and has-miR-mit4. Luciferase assay was used to invent whether MT-RNR2 was the direct target of hsa-miR-mit3 or hsa-miR-mit4 microRNA separately. A wild type and a mutated 3′ UTR of MT-RNR2 gene were subcloned into the psiCHECK-2 luciferase miRNA expression reporter vector as described earlier [23]. The transfected cells were cotransfected with 100 mM anti-hsa-miR-mit3 or the same amount of anti-hsa-miR-mit4.