[Retracted] miR-451a Inhibited Cell Proliferation and Enhanced Tamoxifen Sensitive in Breast Cancer via Macrophage Migration Inhibitory Factor

Figure 3

The expressions of miR-451a and MIF were examined by real-time PCR. (a) total RNA extracted from the cell lines of MCF-7 and LCC2 was subjected to real-time PCR with miR-451a-specific or MIF-specific primers. The miR-451a expression or MIF mRNA expression was normalized to U6 or GAPDH expression. versus MCF-7 cell; (b) total RNA extracted from the stable cell lines of MCF-7 and LCC2 was subjected to real-time PCR with miR-451a-specific primers. The induction of miR-451a expression was normalized to U6 expression; (c) total RNA extracted from these cells was subjected to real-time PCR with MIF-specific primers. The induction of MIF mRNA expression was normalized to GAPDH expression. versus Lv-miR-451a NC (MCF-7); versus Lv-miR-451a NC (LCC2) (note: NC means negative control; Lv means lentivirus).

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