Combined Epidermal Growth Factor Receptor and Beclin1 Autophagic Protein Expression Analysis Identifies Different Clinical Presentations, Responses to Chemo- and Radiotherapy, and Prognosis in Glioblastoma

Figure 4

Double immunofluorescence staining for EGFR (a, d, g, and j; red stain) and Beclin1 (b, e, h, and k; green stain). Representative GBs showing H-EGFR + L-Beclin1 (a, b, and c), L-EGFR + H-Beclin1 (d, e, and f), H-EGFR + H-Beclin1 (g, h, and i), and L-EGFR + L-Beclin1 (j, k, and l) patterns of protein expression. Nuclei are marked by staining with 4,6-diamidino-2-phenylindole (DAPI) and appear blue in the merged pictures (c, f, i, and l). Original magnification ×650. Scale bar = 50 μm.

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