Antiproliferative Activity of Flavonoids from Croton sphaerogynus Baill. (Euphorbiaceae)
Table 1
Major constituents of the crude EtOH extract (EE), MeOH (MP) and hexane (HP) phases, and fractions (F1–F5) from of Croton sphaerogynus leaves, characterized by HPLC-DAD (352 nm) and HPLC-MS.
Constituent
Rt (min)
UV/visible (nm)
Mass spectrum
Suggestion
1
1.75
254, 264 (om), 348
Quercetin derivative
2
2.96
256, 264 (om), 296 (om), 354
757.8; 611.1; 465; 303
755.7
Quercetin triglycoside (2 rhamnose; 1 hexose)
3
3.16
260, 296, 356
741.8; 595.1; 433.1; 286.8
Kaempferol triglycoside (2 rhamnose; 1 hexose)
4
4.05
264, 292, 346
741.8; 595.1; 287
Kaempferol triglycoside (2 rhamnose; 1 hexose)
5
4.30
264, 294, 348
741.9; 595.2; 448.7; 287
739.6
Kaempferol triglycoside (2 rhamnose; 1 hexose)
6
4.99
256, 266 (om), 294 (om), 354
595, 303
Quercetin diglycoside (1 pentose; 1 hexose)
7
5.33
256, 266 (om), 294 (om), 354
661.1; 465
608.9
Rutin
8
6.04
264, 294, 348
595, 286.9
593
Kaempferol diglycoside (1 rhamnose; 1 glucose)
9
6.35
256, 264 (om), 296 (om), 354
479, 316.9
Isorhamnetin monoglycoside (1 hexose)
10
7.30
264, 294, 348
448.9; 286.9
Kaempferol monoglycoside (1 hexose)
11
9.23
264, 294, 346
Kaempferol derivative
12
10.10
256, 268 (om), 356
Quercetin derivative
13
10.72
264, 294, 346
Kaempferol derivative
14
10.97
256, 264 (om), 306 (om), 348
449.0; 302.9
447
Quercitrin
15
20.44
262, 294, 340
Kaempferol derivative
16
22.95
254, 270, 298, 370
Quercetin
23.23
256, 266 (om), 298 (om), 356
317, 302
314.8
Quercetin 3-O-methyl ether
18
23.55
264, 292, 320, 366
Kaempferol
19
23.73
266, 294, 350
Kaempferol derivative
20
23.84
254, 268 (om), 294 (om), 356
Quercetin derivative
Rt: average retention time in minutes. Compound confirmed by 1H NMR.
