Inhibitory Effect of a Novel Antirheumatic Drug T-614 on the IL-6-Induced RANKL/OPG, IL-17, and MMP-3 Expression in Synovial Fibroblasts from Rheumatoid Arthritis Patients

Figure 3

Effect of T-614 and MTX on IL-6-induced RANKL, OPG expression, and the ratio of RANKL/OPG in RASFs. RASFs were stimulated with IL-6/sIL-6R and then treated with T-614 or MTX for 72 h. The effect of T-614 and MTX on the expression of RANKL (a), OPG (d), and RANKL/OPG (e) in IL-6-induced RASFs was tested by real-time quantitative PCR analysis. Total proteins were extracted and expression levels of RANKL protein were determined by western blot (b, c). The data points shown are the mean ± SD for three independent experiments, each in triplicate. ^∗∗∗ and ^∗.

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