A Validated TLC-Densitometric Method for the Determination of Mesterolone in Bulk Material and in Tablets

Table 1

Chromatographic characteristic of mesterolone analyzed using different NP-TLC plates and chloroform-acetone (40 : 10, v/v) as mobile phase.

Mobile phase	Chromatographic plates		Visualization mode
			Densitometric analysis without the use of visualizing agent	Densitometric analysis after derivatizing with 10% sulphuric acid	Densitometric analysis after derivatizing with 10% phosphomolybdic acid (PMA)
			[nm]	[nm]	[nm]
Chloroform-acetone 40 : 10 (v/v)	Silica gel 60; Art. 1.05553; (E. Merck)	0.66 ± 0.01	200	467	745
	Silica gel 60F254; Art. 1.05554; (E. Merck)	0.75 ± 0.02	200	467	745
	Silica gel 60/Kieselguhr F254; Art. 1.05567; (E. Merck)	0.87 ± 0.01	248	468	745