Hypoxia-Induced Epithelial-Mesenchymal Transition Is Involved in Bleomycin-Induced Lung Fibrosis

Figure 1

Bleomycin induces lung fibrosis. (a) A modified Masson trichrome staining. Alveolar septa thickening and cellular infiltration were observed in the lung tissues at 1 week after intratracheal bleomycin injection. (b) Quantitative analysis of fibrosis with Ashcroft scale. (c) Representative Western blots of MMP-2, S100A4, and α-SMA protein. Obvious increases in MMP-2, S100A4, and α-SMA protein expression were observed at 1-week, and progressive increase was observed from 1 to 6 weeks. GAPDH was used as a loading control. (d) Semiquantitative MMP-2 expression from densitometry analysis of bands in (c). (e) Semiquantitative S100A4 expression in (c). (f) Semiquantitative α-SMA expression in (c) (). versus control. .

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