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Figure 4: The evidence of epithelial–mesenchymal transition in cells. H358 cells were treated with hypoxia for 12 h (H-12 h) and 24 h (H-24 h). Control cells were grew up in normal oxygen. (a) Representative Western blots of HIF-1α, ZEB1, and EMT markers in H358 cells. (b) Semiquantitative assay of protein expression in lung tissues in (a). (c) Immunofluorescence staining of S100A4 protein. H358 and Beas-2B cells were treated with 2% O2 for 24 h with or without continuous treatment for 5 days with 10% O2. (d) Representative Western blots of CD44, phospho-p44/42, total p44/42, phospho-p38, and total p38 MAPK in H358 cells. GAPDH was used as a loading control. (e) Semiquantitative assay of protein expression in lung tissues in (c); .