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BioMed Research International
Volume 2015, Article ID 235108, 6 pages
Research Article

Bone Regeneration of Hydroxyapatite/Alumina Bilayered Scaffold with 3 mm Passage-Like Medullary Canal in Canine Tibia Model

1Xenotransplantation Research Center, Department of Microbiology and Immunology, Institute of Endemic Diseases, College of Medicine, Seoul National University, Seoul 110-799, Republic of Korea
2Korea Textile Development Institute, Daegu 703-702, Republic of Korea
3Department of Veterinary Surgery, Chonnam National University, Gwangju 500-757, Republic of Korea
4Veterinary Medical Center, Chungbuk National University, Cheongju 361-763, Republic of Korea

Received 9 May 2014; Accepted 23 October 2014

Academic Editor: Sung-Hoon Kim

Copyright © 2015 Jong Min Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study was to evaluate the bone regeneration of hydroxyapatite (HA)/alumina bilayered scaffold with a 3 mm passage-like medullary canal in a beagle tibia model. A porous HA/alumina scaffold was fabricated using a polymeric template-coating technique. HA/alumina scaffold dimensions were 10 mm in outer diameter, 20 mm in length, and with either a 3 mm passage or no passage. A 20 mm segmental defect was induced using an oscillating saw through the diaphysis of the beagle tibia. The defects of six beagles were filled with HA/alumina bilayered scaffolds with a 3 mm passage or without. The segmental defect was fixated using one bone plate and six screws. Bone regeneration within the HA/alumina scaffolds was observed at eight weeks after implantation. The evaluation of bone regeneration within the scaffolds after implantation in a beagle tibia was performed using radiography, computerized tomography (CT), micro-CT, and fluorescence microscopy. New bone successfully formed in the tibia defects treated with 3 mm passage HA/alumina scaffolds compared to without-passage HA/alumina scaffolds. It was concluded that the HA/alumina bilayered scaffold with 3 mm passage-like medullary canal was instrumental in inducing host-scaffold engraftment of the defect as well as distributing the newly formed bone throughout the scaffold at 8 weeks after implantation.