Effect of 50 Hz Extremely Low-Frequency Electromagnetic Fields on the DNA Methylation and DNA Methyltransferases in Mouse Spermatocyte-Derived Cell Line GC-2

Figure 3

Differentially methylated genes verified with methylation-specific PCR and real-time PCR in 50 Hz ELF-EMF exposure. (a) Representative MSP results of the three genes (Fut11, Olfr969A, and Tagln) methylation in GC-2 cells at magnetic field intensity of 1.0 mT. M: methylated primers; U: unmethylated primers. (b) Validation of mRNA expression of the three genes (Fut11, Olfr969A, and Tagln) by real-time PCR. (c) Representative MSP results of the three genes (Fut11, Olfr969B, and Lrrc9) methylation in GC-2 cells at magnetic intensity of 3.0 mT. (d) Validation of mRNA expression of the three genes (Fut11, Olfr969B, and Lrrc9) by real-time PCR.

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