Docosahexaenoic Acid Induces Cell Death in Human Non-Small Cell Lung Cancer Cells by Repressing mTOR via AMPK Activation and PI3K/Akt Inhibition

Figure 3

DHA-induced autophagy is required for apoptotic cell death. The indicated cancer cell lines were treated with nontargeting control small interfering RNA (siNC) or siRNAs specific for autophagy-related Atg5 (a) and Atg7 (b). At 18 h after transfection, cells were incubated with the indicated doses of DHA for 24 h. Next, cells were harvested and subjected to western blot analysis with the following antibodies: Atg5, Atg7, LC3-II, and actin (upper panel). Cell viability was measured in an MTT assay (lower panel). and . Data are representative of three independent experiments, all with similar results.

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