Use of Dried Plasma Spots for HIV-1 Viral Load Determination and Drug Resistance Genotyping in Mexican Patients
Table 1
Rate of HIV pol sequences PCR amplification from plasma and DPS paired samples.
ID
Time of DPS storage (days)
VL (log10 copies/mL)
PCR amplification
DR genotype
Plasma
DPS
Plasma
DPS
Plasma
DPS
HIV-47
3
6.2
6.1
HIV-03
7
6.1
4.6
HIV-43
7
5.4
4.8
HIV-08
7
5.3
4.7
HIV-86
7
5.2
4.5
HIV-31
7
5.1
4.9
HIV-55
7
5.0
4.7
HIV-64
7
5.0
4.4
HIV-88
12
5.0
4.4
HIV-20
7
5.0
4.5
HIV-13
12
4.9
4.9
HIV-18
7
4.8
4.0
HIV-54
7
4.7
3.8
HIV-89
7
4.5
3.6
HIV-66
7
4.3
3.5
HIV-69
7
4.3
3.7
HIV-62
7
4.3
3.5
HIV-39
7
4.2
3.5
HIV-04
7
4.9
4.0
HIV-02
7
3.5
3.1
HIV-01
7
2.1
1.7
HIV-22
910
4.4
4.5
Average SD
4.8 0.9
4.2 0.9
14/22
14/22
14/22
13/22
Rate of PCR amplification and genotyping (%)
63.6
59.1
VL in DPS and liquid plasma using Amplicor HIV-1 Monitor Test, version 1.5. Stanford genotyping drug resistance interpretation algorithm (v.4.2.6) (http://sierra2.stanford.edu/sierra/servlet/JSierra). DR: drug resistance genotype. HIV-DPS sample stored for 910 days.
