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BioMed Research International
Volume 2015 (2015), Article ID 241582, 6 pages
http://dx.doi.org/10.1155/2015/241582
Research Article

Fungal Antagonism Assessment of Predatory Species and Producers Metabolites and Their Effectiveness on Haemonchus contortus Infective Larvae

1Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brazil
2Universidade Vila Velha, Vila Velha, ES, Brazil
3Facultad de Ciencias Agropecuárias, UAEM, Avenida Universidad 1001, Col. Chamilpa, 62209 Cuernavaca, MOR, Mexico
4Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias INIFAP/CENID, Avenida Progreso No. 5, Colonia Barrio de Santa Catarina, 04010 Delegación Coyoacán, DF, Mexico
5Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, Brazil

Received 13 April 2015; Accepted 21 July 2015

Academic Editor: Giuseppe Piccione

Copyright © 2015 Manoel Eduardo Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The objective of this study was to assess antagonism of nematophagous fungi and species producers metabolites and their effectiveness on Haemonchus contortus infective larvae (L3). Assay A assesses the synergistic, additive, or antagonistic effect on the production of spores of fungal isolates of the species Duddingtonia flagrans, Clonostachys rosea, Trichoderma esau, and Arthrobotrys musiformis; Assay B evaluates in vitro the effect of intercropping of these isolates grown in 2% water-agar (2% WA) on L3 of H. contortus. D. flagrans (Assay A) produced 5.3 × 106 spores and associated with T. esau, A. musiformis, or C. rosea reduced its production by 60.37, 45.28, and 49.05%, respectively. T. esau produced 7.9 × 107 conidia and associated with D. flagrans, A. musiformis, or C. rosea reduced its production by 39.24, 82.27, and 96.96%, respectively. A. musiformis produced 7.3 × 109 spores and associated with D. flagrans, T. esau, or C. rosea reduced its production by 99.98, 99.99, and 99.98%, respectively. C. rosea produced 7.3 × 108 conidia and associated with D. flagrans, T. esau, or A. musiformis reduced its production by 95.20, 96.84, and 93.56%, respectively. These results show evidence of antagonism in the production of spores between predators fungi.