Diverse Reductive Dehalogenases Are Associated with Clostridiales-Enriched Microcosms Dechlorinating 1,2-Dichloroethane

Table 1

Summary of the OTU representatives, identified in the 16S rRNA gene libraries.

OTU	Clones		Basic Local Alignment Search Tool-GenBank			CLASSIFIER Match Tool-Ribosomal Database Project II
			Closest described relative	Acc.	% identity	Phylogenetic group	Closest classified relative	% certainty^A
1	3	0	Geobacter thiogenes	NR_028775	98.96	Deltaproteobacteria	Geobacter	100
2	3	0	Geobacter thiogenes	NR_028775	98.78	Deltaproteobacteria	Geobacter	100
3	8	0	Geobacter thiogenes	NR_028775	99.09	Deltaproteobacteria	Geobacter	100
4	3	0	Unc. bacterium	AM410013	97.34	Deltaproteobacteria	Geobacter	100
5	1	0	Unc. Geobacter sp.	FM204959	98.63	Deltaproteobacteria	Geobacter	100
6	1	0	Unc. Geobacter sp.	EU266833	98.62	Deltaproteobacteria	Geobacter	100
7	3	0	Unc. Geobacter sp.	AY752765	98.56	Deltaproteobacteria	Geobacter	100
8	1	0	Unc. Geobacter sp.	AY752765	98.42	Deltaproteobacteria	Geobacter	100
9	1	0	Unc. Dehalobacter sp.	HM748813	99.43	Clostridia	Acetobacterium	100
10	4	0	Unc. Sulfurimonas sp.	KF851122	98.34	Epsilonproteobacteria	Sulfuricurvum	100
11	4	0	Ferribacterium sp. 7A-631	KF441656	99.75	Betaproteobacteria	Ferribacterium	93
12	1	0	Unc. Gallionellaceae bacterium	EU266776	96.48	Proteobacteria	Betaproteobacteria	100
13	1	0	Unc. Rhodocyclaceae bacterium	JQ279024	98.83	Betaproteobacteria	Rhodocyclaceae	98
14	1	0	Unc. Rhodocyclaceae bacterium	HQ003471	97.64	Betaproteobacteria	Rhodocyclaceae	100
15	0	1	Acinetobacter baumannii	KJ958271	99.60	Gammaproteobacteria	Acinetobacter	100
16	0	1	Pseudomonas putida	GU396283	98.97	Gammaproteobacteria	Pseudomonas	100
17	0	7	Unc. Bacteroides sp.	AB529592	99.44	Bacteroidia	Parabacteroides	99
18	0	1	Unc. Bacteroidetes bacterium	FJ535139	98.31	Bacteroidia	Parabacteroides	100
19	0	1	Unc. Bacteroides sp.	JQ624314	99.75	Bacteroidia	Parabacteroides	100
20	0	1	Unc. Bacteroidetes bacterium	DQ676360	98.97	Bacteroidia	Porphyromonadaceae	99
21	0	1	Unc. Bacteroides sp.	FM204969	99.88	Bacteroidia	Parabacteroides	99
22	0	1	Unc. Acidaminobacter sp.	HM217344	98.61	Clostridia	Clostridiales Incertae Sedis XII	91
23	0	6	Unc. Acidaminobacter sp.	HM217344	98.78	Clostridia	Clostridiales	100
24	0	5	Unc. Acidaminobacter sp.	HM217344	99.46	Clostridia	Clostridiales Incertae Sedis XII	80
25	2	0	Unc. Hydrogenophaga sp.	HM124825	99.67	Betaproteobacteria	Hydrogenophaga	100
26	3	0	Hydrogenophaga taeniospiralis	AY771764	98.02	Betaproteobacteria	Hydrogenophaga	100
27	0	7	Malikia spinosa	NR_040904	99.86	Betaproteobacteria	Malikia	100
28	1	0	Unc. Hydrogenophaga sp.	DQ413154	98.70	Betaproteobacteria	Hydrogenophaga	100
29	1	0	Unc. Elusimicrobia bacterium	GU236016	94.55	Elusimicrobia	Elusimicrobium	98
30	0	2	Hydrogenophaga taeniospiralis	AY771764	98.75	Betaproteobacteria	Hydrogenophaga	95
31	8	0	Hydrogenophaga taeniospiralis	AY771764	99.06	Betaproteobacteria	Hydrogenophaga	98
32	0	6	Malikia spinosa	NR_040904	99.73	Betaproteobacteria	Malikia	88
33	1	0	Unc. Acidovorax sp.	AM084039	99.04	Betaproteobacteria	Comamonadaceae	100
34	3	1	Unc. Dechloromonas sp.	JN679130	98.95	Betaproteobacteria	Rhodocyclaceae	100
35	1	0	Unc. Gallionella sp.	FJ391502	98.72	Proteobacteria	Betaproteobacteria	100
36	0	2	Vogesella indigofera	NR_040800	99.60	Betaproteobacteria	Vogesella	100
37	0	1	Shewanella putrefaciens	JN019028	99.87	Gammaproteobacteria	Shewanella	100
38	9	0	Sulfuricurvum kujiense	CP002355	99.22	Epsilonproteobacteria	Sulfuricurvum	100
39	1	0	Unc. Arcobacter sp.	JQ861849	97.96	Epsilonproteobacteria	Arcobacter	93
40	2	0	Geobacter metallireducens	NR_075011	98.31	Deltaproteobacteria	Geobacter	100
41	3	0	Unc. Geobacter sp.	EU266817	99.76	Deltaproteobacteria	Geobacter	100
42	2	0	Unc. Geobacter sp.	EU266841	99.16	Deltaproteobacteria	Geobacter	100
43	5	0	Geobacter lovleyi	NR_074979	99.03	Deltaproteobacteria	Geobacter	100
44	1	0	Geobacter thiogenes	NR_028775	97.48	Deltaproteobacteria	Geobacter	100
45	1	1	Unc. Firmicutes bacterium	HQ003641	98.70	Clostridia	Clostridiales Incertae Sedis XII	100
46	0	20	Unc. Firmicutes bacterium	HQ003641	99.45	Clostridia	Acidaminobacter	86
47	0	1	Unc. Clostridium sp.	FM204998	100.0	Clostridia	Clostridium XlVa	100
48	0	3	Acetobacterium malicum	NR_026326	99.53	Clostridia	Acetobacterium	100
49	1	0	Unc. bacterium	AB759668	95.24	Bacteria	Firmicutes	100
50	2	0	Unc. rumen bacterium	AB615047	94.24	Lentisphaerae	Victivallis	97
51	2	0	Unc. Cytophaga sp.	EU809766	99.35	Lentisphaerae	Victivallis	97
52	1	0	Denitrifying bacterium	FJ802233	98.54	Ignavibacteria	Ignavibacterium	91
53	0	8	Unc. Bacteroides sp.	FJ862827	99.18	Bacteroidia	Parabacteroides	100
54	0	3	Macellibacteroides fermentans	NR_117913	99.08	Bacteroidia	Parabacteroides	99
55	0	1	Unc. Bacteroidetes bacterium	FJ535139	94.64	Bacteroidia	Porphyromonadaceae	88
56	0	1	Unc. Bacteroidetes bacterium	DQ676360	99.30	Bacteroidia	Bacteroidales	99
57	1	0	Unc. Prolixibacter sp.	JQ723616	97.85	Bacteria	Bacteroidetes	100
58	1	0	Unc. Geobacter sp.	JQ086897	98.72	Deltaproteobacteria	Geobacter	100
59	1	0	Geobacter lovleyi	NR_074979	99.37	Deltaproteobacteria	Geobacter	100
60	1	0	Sulfuricurvum kujiense	NR_074398	99.29	Epsilonproteobacteria	Sulfuricurvum	100

Confidence threshold of the RDPII CLASSIFIER Tool is 80%.