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BioMed Research International
Volume 2015, Article ID 243173, 7 pages
Research Article

Assessment of Morphological and Functional Changes in Organs of Rats after Intramuscular Introduction of Iron Nanoparticles and Their Agglomerates

1Orenburg State University, Prospekt Pobedy 13, Orenburg 460018, Russia
2State Educational Institution, All-Russian Research Institute of Beef Cattle Breeding, RAAS, No. 29, 9 Yanvarya Street, Orenburg 460018, Russia
3Orenburg State Medical Academy of Federal Agency in Public Health and Social Development, 6 Sovetskaya Street, Orenburg 460000, Russia

Received 12 November 2014; Revised 30 January 2015; Accepted 30 January 2015

Academic Editor: Goutam Ghosh Choudhury

Copyright © 2015 Elena Sizova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The research was performed on male Wistar rats based on assumptions that new microelement preparations containing metal nanoparticles and their agglomerates had potential. Morphological and functional changes in tissues in the injection site and dynamics of chemical element metabolism (25 indicators) in body were assessed after repeated intramuscular injections (total, 7) with preparation containing agglomerate of iron nanoparticles. As a result, iron depot was formed in myosymplasts of injection sites. The quantity of muscle fibers having positive Perls’ stain increased with increasing number of injections. However, the concentration of the most chemical elements and iron significantly decreased in the whole skeletal muscle system (injection sites are not included). Consequently, it increased up to the control level after the sixth and the seventh injections. Among the studied organs (liver, kidneys, and spleen), Caspase-3 expression was revealed only in spleen. The expression had a direct dependence on the number of injections. Processes of iron elimination from preparation containing nanoparticles and their agglomerates had different intensity.