After Myocardial Ischemia-Reperfusion, miR-29a, and Let7 Could Affect Apoptosis through Regulating IGF-1

Figure 4

MiR-29a and Let7f influenced IGF-1 downstream related apoptosis pathway. (a) H9C2 cell apoptosis was detected by TUNEL assay. (b) We transfected cell with mimic-miR-29a and mimic-Let7f, then found the protein level of p-Akt and Bcl-2 decreased while caspase-3 and Bax increased. (c) After transfection with anti-miR-29a and anti-Let7f, Western blot results showed that p-Akt and Bcl-2 significantly increased, while caspase-3 and Bax protein significantly decreased.

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