Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2015 (2015), Article ID 247965, 6 pages
http://dx.doi.org/10.1155/2015/247965
Research Article

Evaluation of Hemagglutination Activity of Chitosan Nanoparticles Using Human Erythrocytes

1Grupo de Estudos e Pesquisas em Imunologia Humana (GEPIH), Escola Tecnica de Saúde da UFPB, Universidade Federal da Paraíba, 58051-900 João Pessoa, PB, Brazil
2Departamento de Fisiologia e Patologia, Centro de Ciencias da Saúde, Universidade Federal da Paraíba, 58051-900 João Pessoa, PB, Brazil
3Centro de Pesquisas Aggeu Magalhães (CPqAM/FIOCRUZ) e Laboratório de Imunopatologia Keio Asami (LIKA), Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil
4Departamento de Engenharia de Materiais, Centro de Tecnologia, Universidade Federal da Paraíba, 58051-900 João Pessoa, PB, Brazil
5Núcleo de Biomateriais, NEPBIO, Centro de Ciências da Saúde, Universidade Federal da Paraíba, 58051-900 João Pessoa, PB, Brazil
6Departamento de Engenharia, Universidade Federal de Lavras, 37200-000 Lavras, MG, Brazil
7Departamento de Odontologia Clínica e Social, Centro de Ciencias da Saúde, Universidade Federal da Paraíba, 58051-900 João Pessoa, PB, Brazil

Received 10 October 2014; Revised 20 January 2015; Accepted 21 January 2015

Academic Editor: Jianshu Li

Copyright © 2015 Jefferson Muniz de Lima et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Chitosan is a polysaccharide composed of randomly distributed chains of β-(1-4) D-glucosamine and N-acetyl-D-glucosamine. This compound is obtained by partial or total deacetylation of chitin in acidic solution. The chitosan-based hemostatic agents have been gaining much attention in the management of bleeding. The aim of this study was to evaluate in vitro hemagglutination activity of chitosan nanoparticles using human erythrocytes. The preparation of nanoparticles was achieved by ionotropic gelification technique followed by neutralization with NaOH 1 mol/L−1. The hemagglutination activity was performed on a solution of 2% erythrocytes (pH 7.4 on PBS) collected from five healthy volunteers. The hemolysis determination was made by spectrophotometric analysis. Chitosan nanoparticle solutions without NaOH addition changed the reddish colour of the wells into brown, suggesting an oxidative reaction of hemoglobin and possible cell lysis. All neutralized solutions of chitosan nanoparticles presented positive haemagglutination, without any change in reaction color. Chitosan nanoparticles presented hemolytic activity ranging from 186.20 to 223.12%, while neutralized solutions ranged from 2.56 to 72.54%, comparing to distilled water. Results highlight the need for development of new routes of synthesis of chitosan nanoparticles within human physiologic pH.