Evaluation of Hemagglutination Activity of Chitosan Nanoparticles Using Human Erythrocytes

Table 1

Percentage of hemagglutination reactivity of human erythrocytes incubated with chitosan nanoparticles.

Solution mg/mL	Hemagglutination (%)
	++++	+++	++	+	±	Indeterminate
	Solution A 0.4	—	—	—	—	—	100
Solution A 1.0	—	—	—	—	—	100
Solution A 1.6	—	—	—	—	—	100
Solution A 3.0	—	—	—	—	—	100
Solution A 0.4	100	—	—	—	—	—
Solution A 1.0	100	—	—	—	—	—
Solution A 1.6	100	—	—	—	—	—
Solution A 3.0	100	—	—	—	—	—
Solution B 0.4	—	—	—	—	—	100
Solution B 1.0	—	—	—	—	—	100
Solution B 1.6	—	—	—	—	—	100
Solution B 3.0	—	—	—	—	—	100
Solution B 0.4	—	—	—	100	—	—
Solution B 1.0	—	80	—	—	20	—
Solution B 1.6	—	90	10	—	—	—
Solution B 3.0	—	100	—	—	—	—

Percentage of the wells compatible with Stavitsky (1954) [18].
Indicates the percentage of the samples that showed color modification that distorted the visual definition of the hemagglutinating event.
Neutralized solution by the addition of NaOH 1 mol/L⁻¹.