Evaluation of Hemagglutination Activity of Chitosan Nanoparticles Using Human Erythrocytes

Table 2

Hemolytic activity of the diverse chitosan nanoparticle formulations in human erythrocytes.

Solutions	Optical density; 560 nm	Hemolysis %
Distilled water	0.137 ± 0.032	+control
NaCl 0.9% saline solution	0.034 ± 0.009	−control
Solution A 0.4 mg/mL	0.226 ± 0.024	186.20
Solution A 1 mg/mL	0.237 ± 0.014	197.16
Solution A 1.6 mg/mL	0.234 ± 0.021	194.07
Solution A 3 mg/mL	0.252 ± 0.026	212.02
Solution A neutralized 0.4 mg/mL	0.037 ± 0.004	2.56
Solution A neutralized 1 mg/mL	0.047 ± 0.018	12.97
Solution A neutralized 1.6 mg/mL	0.045 ± 0.007	10.44
Solution A neutralized 3 mg/mL	0.059 ± 0.011	23.95
Solution B 0.4 mg/mL	0.228 ± 0.018	188.72
Solution B 1 mg/mL	0.234 ± 0.018	193.91
Solution B 1.6 mg/mL	0.238 ± 0.020	198.46
Solution B 3 mg/mL	0.264 ± 0.027	223.12
Solution B neutralized 0.4 mg/mL	0.088 ± 0.124	52.48
Solution B neutralized 1 mg/mL	0.052 ± 0.010	17.56
Solution B neutralized 1.6 mg/mL	0.109 ± 0.109	72.54
Solution B neutralized 3 mg/mL	0.063 ± 0.010	28.19