Codon Optimization Significantly Improves the Expression Level of α-Amylase Gene from Bacillus licheniformis in Pichia pastoris

Figure 3

-Amylase activity and total protein content in basal salt medium at 30°C and pH 5.0 during fed-batch fermentation in 5-L (a) and 50-L (b) bioreactor. -Amylase activity was determined by starch-iodine color method; the protein content was determined according to the Bradford method using BSA as standard. All measurements were carried out in triplicate.

(a)

(b)