Acupuncture May Exert Its Therapeutic Effect through MicroRNA-339/Sirt2/NFB/FOXO1 Axis
Sirt2 is regulated by miR-339 directly. (a) Sequence of human miR-339 and the predicted binding sites with miR-339 within Sirt2 untranslated region (3′-UTR) from different species are shown. The sequence of Sirt2 3′-UTR mutant used for reporter assay is also shown. (b) Luciferase reporter constructs containing 3′-UTR (Luc-Sirt2-3′-UTR) or mutant 3′-UTR (Luc-Sirt2-3′-UTR-mut) of Sirt2 gene were cotransfected with miR-339 mimic or empty vector in SH-SY5Y cell line and the luciferase activities were assayed (, versus control).