SERCA2 Haploinsufficiency in a Mouse Model of Darier Disease Causes a Selective Predisposition to Heart Failure

Figure 2

Effects of Atp2a2 heterozygosity on expression of Ca²⁺ handling proteins in heart. Adult WT and Atp2a2^+/− (HET) hearts were processed for analysis of mRNA and protein levels. (a) Atp2a2 mRNA determined by RT-PCR; (b) immunoblot analysis of SERCA2a, ryanodine receptor isoform 2 (RYR2), 2 subunit of L-type Ca²⁺ channel (LTCC2), and Na⁺/Ca²⁺ exchanger isoform 1 (NCX1). Quantitation of SERCA2a (c) and LTCC2 (d) protein levels. Immunoblot analyses of phospholamban (PLN) and PLN phosphorylated on Ser16 (PS16) and Thr17 (PT17) were performed using heart samples from anesthetized surgically instrumented mice under both baseline conditions (e) and after -adrenergic stimulation with dobutamine at 16 ng/g body weight/min (f, g, h). mRNA levels were normalized to Gapdh and protein levels were normalized to sarcomeric actin (s.actin). Values are means ± SE. = at least 4 for each genotype. versus WT controls.

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