Roles of ER and GPR30 in Proliferative Response of Human Bladder Cancer Cell to Estrogen

Figure 2

GPR30 mediated an inhibitory effect in T24 cells. (a) T24 cells were transfected with 10 nM specific siRNA against ERβ, and then protein expression levels were measured by western blot. (b) ERβ mRNA expression levels by qPCR analysis. The level of ERβ in control cells was defined as 1.0. (c) E2 inhibited T24 cell proliferation in the absence of ERβ. Cells were transfected with siRNA against ERβ or ERβ ORF expression vector and then treated with 10 nM E2 for 0–96 h. MTT assays were performed to measure the cell activity. (d) Cell proliferation was inhibited by E2-BSA. T24 cells were treated as shown in Figure 1(c), and MTT assays were used to monitor the effect of E2-BSA. (e) T24 cells were transfected with siRNA against GPR30. The cells were treated as shown in (a), and protein levels were measured by western blot analysis. (f) Expression levels of GPR30 mRNA by qPCR analysis. The level of GPR30 was defined as 1.0. (g) GPR30 mediated inhibition of T24 cell proliferation. The cells were transfected with 10 nM siRNA against GPR30 or GPR30 ORF expression vector and then treated with 10 nM E2 or 10 nM E2-BSA for 0–96 h. MTT assays were used to detect cell activity. The data presented here is one typical experiment from three independent experiments. ; .

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