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Region | Laboratory/year of publication | Number of isolated NTM | Detection | Identification |
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| Mycobacteriology Research Center (MRC)-NRIT LD-2014 [51] | Clinical isolates: 124 | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Amplification of IS6110 and PCR-RFLP for hsp65 |
| Mycobacteriology Research Center (MRC)-NRIT LD-2013 [52] | Environmental isolates (RGM): 36; total: 193 | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Amplification of IS6110 and PCR RFLP of hsp65 and 16 s–23 s rRNA |
Tehran | Department of Mycobacteriology, Pasteur Institute of Iran-2013 [2] | Clinical isolates: 32 | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation, biochemical properties, and PCR based on hsp65 |
Masoud Laboratory, TB Reference Centre of Ahvaz and Kermanshah, 2013 [54] | Clinical isolates: 23 | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation, biochemical properties, amplification, sequencing of 16S rRNA, rpoB, hsp65, and ITS (Internal Transcribed Spacer) |
| Masoud Laboratory-2012 [13] | Clinical isolates: 63 | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation, biochemical properties, amplification of IS6110, and PCR based on hsp65 |
| Mycobacteriology Research Center (MRC)-NRIT LD-2009 [14] | Clinical isolates: 43 | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation, biochemical properties, and PCR-RFLP for hsp65 |
|
| Dept. of Microbiology, Isfahan University of Medical Sciences-2014 [15] | Clinical isolates: 34 | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Amplification and sequencing of 16SrRNA and RFLP-PCR for hsp65 |
| Dept. of Microbiology, Isfahan University of Medical Sciences-2013 [16] | Clinical isolates: 21 | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation and biochemical properties |
Isfahan | Dept. of Microbiology, Isfahan University of Medical Sciences-2012 [17] | Environmental isolates: 21 | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation and biochemical properties |
Dept. of Microbiology, Isfahan University of Medical Sciences-2012 [18] | Environmental isolates: 22 | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation and biochemical properties |
| Isfahan University of Medical Sciences-2011 [4] | Clinical isolates: 67 | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation, biochemical properties, amplification, sequencing of 16SrRNA, and RFLP-PCR for hsp65 |
| Isfahan University of Medical Sciences-2010 [19] | Clinical isolates: RGM:25 | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation, biochemical properties, genus and species specific PCR, and PCR based on hsp65 |
|
Golestan | Health care centers of Golestan province-2013 [20] | Clinical isolates: 19 | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation, biochemical properties, amplification, and sequencing of 16S rRNA |
Microbiology Laboratory of Urmia University of Medical sciences-2006 [21] | Environmental isolates: 161 | Culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation and biochemical properties |
|
Khuzestan | Tuberculosis reference laboratory, PHLS of Khuzestan province-2009 [22] | Clinical isolates: 8 | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation, biochemical properties, and PCR based on hsp65 |
Ahwaz University of Medical Sciences-1995 [23] | Clinical isolates: 18 | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation and biochemical properties |
|
West Azerbaijan | Microbiology Laboratory of Urmia University of Medical Sciences-2010 [3] | Environmental isolates: 65 | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation and biochemical properties |
|
Gilan | 1992 [24] | Environmental isolates: 33 | Culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation and biochemical properties |
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Sistan-Baluchestan | 2006 [25] | Clinical: 3 NTM identified | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation and biochemical properties |
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Kerman | 2007 [26] | Clinical NTM: 3 NTM identified | Smear microscopy and culture on solid media (Löwenstein-Jensen) | Growth characteristics and pigmentation and biochemical properties |
|