Table 1: Detection and identification procedures for nontuberculous mycobacteria (NTM) in different laboratories.

RegionLaboratory/year of publicationNumber of isolated NTMDetectionIdentification

Mycobacteriology Research Center (MRC)-NRIT LD-2014 [51]Clinical isolates: 124Smear microscopy and culture on solid media (Löwenstein-Jensen)Amplification of IS6110 and PCR-RFLP for hsp65
Mycobacteriology Research Center (MRC)-NRIT LD-2013 [52]Environmental isolates (RGM): 36; total: 193Smear microscopy and culture on solid media (Löwenstein-Jensen)Amplification of IS6110 and PCR RFLP of hsp65 and 16 s–23 s  rRNA
TehranDepartment of Mycobacteriology, Pasteur Institute of Iran-2013 [2]Clinical isolates: 32Smear microscopy and culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation, biochemical properties, and PCR based on hsp65
Masoud Laboratory, TB Reference Centre of Ahvaz and Kermanshah, 2013 [54]Clinical isolates: 23Smear microscopy and culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation, biochemical properties, amplification, sequencing of 16S rRNA, rpoB, hsp65, and ITS (Internal Transcribed Spacer)
Masoud Laboratory-2012 [13]Clinical isolates: 63Smear microscopy and culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation, biochemical properties, amplification of IS6110, and PCR based on hsp65
Mycobacteriology Research Center (MRC)-NRIT LD-2009 [14]Clinical isolates: 43Smear microscopy and culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation, biochemical properties, and PCR-RFLP for hsp65

Dept. of Microbiology, Isfahan University of Medical Sciences-2014 [15]Clinical isolates: 34Smear microscopy and culture on solid media (Löwenstein-Jensen)Amplification and sequencing of 16SrRNA and RFLP-PCR for hsp65
Dept. of Microbiology, Isfahan University of Medical Sciences-2013 [16]Clinical isolates: 21Smear microscopy and culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation and biochemical properties
IsfahanDept. of Microbiology, Isfahan University of Medical Sciences-2012 [17]Environmental isolates: 21Smear microscopy and culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation and biochemical properties
Dept. of Microbiology, Isfahan University of Medical Sciences-2012 [18]Environmental isolates: 22Smear microscopy and culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation and biochemical properties
Isfahan University of Medical Sciences-2011 [4]Clinical isolates: 67Smear microscopy and culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation, biochemical properties, amplification, sequencing of 16SrRNA, and RFLP-PCR for hsp65
Isfahan University of Medical Sciences-2010 [19]Clinical isolates: RGM:25Smear microscopy and culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation, biochemical properties, genus and species specific PCR, and PCR based on hsp65

GolestanHealth care centers of Golestan province-2013 [20]Clinical isolates: 19Smear microscopy and culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation, biochemical properties, amplification, and sequencing of 16S rRNA
Microbiology Laboratory of Urmia University of Medical sciences-2006 [21]Environmental isolates: 161Culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation and biochemical properties

KhuzestanTuberculosis reference laboratory, PHLS of Khuzestan province-2009 [22]Clinical isolates: 8Smear microscopy and culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation, biochemical properties, and PCR based on hsp65
Ahwaz University of Medical Sciences-1995
[23]
Clinical isolates: 18Smear microscopy and culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation and biochemical properties

West AzerbaijanMicrobiology Laboratory of Urmia University of Medical Sciences-2010 [3]Environmental isolates: 65Smear microscopy and culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation and biochemical properties

Gilan1992 [24]Environmental isolates: 33Culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation and biochemical properties

Sistan-Baluchestan2006 [25]Clinical: 3 NTM identifiedSmear microscopy and culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation and biochemical properties

Kerman2007 [26] Clinical NTM: 3 NTM 
identified
Smear microscopy and culture on solid media (Löwenstein-Jensen)Growth characteristics and pigmentation and biochemical properties