Neuroprotection in Preterm Infants

Figure 3

(a) Oligodendrocyte precursor cells between the 3rd and 9th day in culture (d3–d9). The y-axis shows the number of cells per field of vision. The administration of INF- (100 U/mL) and TNF- (100 ng/mL) severely reduced the number of surviving cells (). Western blot was conducted for MBP, MAG, and CNP. On the 12th day in culture pronounced expression of MBP, MAG, and CNP was observed, indicating the differentiation of the oligodendrocyte precursor into the mature cell type. The administration of IFN- and TNF- almost completely inhibits the expression of these proteins [38]. IFN- = interferon gamma; TNF- = tumor necrosis factor-alpha; MBP = myelin-basic protein; MAG = myelin associated protein; CNP = 2^′,3^′-cyclic nucleotide 3^′-phosphodiesterase.

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